Journal: Animal Cells and Systems
Article Title: Tortoise-shell glue ameliorates male infertility through the hypothalamic-pituitary-gonadal axis in obese rats
doi: 10.1080/19768354.2024.2329684
Figure Lengend Snippet: Figure 2. Effects of TSG treatment on HPG axis in obese rats with male infertility. (A) The mRNA levels of GnRH, RFRP-3, and kisspeptin in the hypothalamus tissues of the four groups using qRT-PCR. (B) The mRNA levels of GnRHR, RFRP-3, and kisspeptin in the pituitary tissues of the four groups using qRT-PCR. (C) Expression levels of LH, FSH, PRL, E2, PROG, and T in the serum of the four groups using ELISA. (D) Expression levels of GnRH and leptin in the serum of the four groups using ELISA. Data are expressed as mean ± SD (n = 5). *P < 0.05 vs. Control group, #P < 0.05 vs. HFD + CTX group. HFD, high-fat diet; CTX, Cyclophosphamide; TSG, tortoise-shell glue; GnRH, gonadotropin-releasing hormone; RFRP-3, RF amide-related peptide-3; GnRHR, GnRH receptor; LH, luteinizing hormone; FSH, follicle-stimulating hormone; PRL, prolactin; E2, estradiol; PROG, progesterone; T, testosterone.
Article Snippet: The concentrations of luteinizing hormone (LH), follicle- stimulating hormone (FSH), prolactin (PRL), estradiol (E2), progesterone (PROG), T, GnRH, and leptin in the serum were detected using the rat LH kit (CSBE12654r, CUSABIO, Wuhan, China), rat FSH kit (E-ELR0391c, Elabscience, Wuhan, China), rat PRL kit (CSBE06881r, CUSABIO), rat E2 kit (CSB-E05110r, CUSABIO), rat PROG kit (CSB-E07282r, CUSABIO), rat T kit (CSBE05100r, CUSABIO), rat GnRH kit (CSB-E08037r, CUSABIO), and rat leptin kit (CSB-E07433r, CUSABIO) following the manufacturers’ instructions, respectively.
Techniques: Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay, Control